From scimedx diagnostic.
6 well x 10 slides = 60 tests.
Positive: A positive result is reported when the cytoplasm of the human granulocyte substrate displays a 1 + or greater fluorescence. P-ANCA and C-ANCA will give a similar uneven granular staining of the cytoplasm, with formalin fixation. C-ANCA and P-ANCA may occur together C-ANCA antibodies are associated with classic Wegener's granulomatosis. P-ANCA (MPO) antibodies are associated with renal limited diseases.
Negative: A serum is considered negative for ANCA if the cytoplasm fluorescence is less then 1 +. Patients should be screened on ANA HEp2 (Cat. 1120L) substrate to avoid confusion with PSEUDO-ANCA. PSEUDO-ANCA will stain the cytoplasm of HEp2 cells whereas true ANCA will be negative on HEp2 unless the patient possesses both ANA and ANCA antibodies.