From scimedx diagnostic.
6 well x 10 slide.
Positive = C-ANCA is identified as a positive result when there is intense positive granular staining of the cytoplasm that extends to the border of the human granulocyte substrate displaying a 1+ or greater fluorescence and there is absence of nuclear staining.
Negative = A serum is considered negative for ANCA if there is an absence or minimal staining of the cytoplasm, or a low level of uniform (not granular) staining of the cytoplasm. The fluorescence is less than 1+ at 1:20 dilution. The multi-lobed nucleus should also be negative.
Sensitivity = 75.7%
Specificity = 86.4%
Agreement = 84.8%